Resources

Protocols

Bench procedures we use routinely and are happy to share.

Software & tools

Open-source tools written in the lab, plus resources we maintain for the wider community.

ProteinCHAOS

MD-inspired generative art tool for visualising protein dynamics.

External ↗

PDB Align

Superpose protein structures and map where they differ, residue by residue.

External ↗

fastPISA

Local interface analysis of PDB and mmCIF complexes — buried surface area, ΔG, and contacts.

External ↗

Bio-Bench

Everyday lab calculators and sequence tools in the browser, offline and with the formulas shown.

External ↗

Infrastructure we use

External platforms and services supporting our daily research and lab operations.

Zulip

Our lab communication is hosted on Zulip Cloud Standard, an organized team chat app designed for efficient communication.

External ↗

Core Facilities at CU Anschutz

Our structural and biophysical work is supported by the School of Medicine's shared Structural Biology and Biophysics core facilities. We routinely access the resources below — follow each link for instrumentation details, rates, and booking.

Core Facility

Cryo-EM Structural Biology Shared Resource

Single-particle cryo-EM from grid screening through automated high-resolution data collection, with remote microscope control.

  • Talos Arctica 200 kV X-FEG (Autoloader)
  • Gatan K3 Summit direct detector
  • Talos L120C 120 kV screening TEM
  • CETA CMOS camera
  • Vitrobot Mark IV
  • Gatan Solarus 950 plasma cleaner
  • Leginon · Appion · cryoSPARC Live
Core Facility

X-Ray Crystallography

Crystallisation screening and optimisation, in-house diffraction data collection, processing, and structure determination.

  • Rigaku MicroMax-007 HF microfocus source
  • VariMax optics · AFC11 goniometer
  • Pilatus 200K area detector
  • Oxford Cobra cryo-system
  • SPT Labtech mosquito Xtal-3 · Phoenix · Alchemist
  • Minstrel imaging · CrystalTrak
  • HKL-3000R
Core Facility

Biophysics

Binding, thermodynamics, oligomeric state, and single-molecule characterisation of proteins and complexes.

  • Biacore T200 (SPR)
  • MicroCal iTC200 (ITC)
  • Monolith NT.115 Pico (MST)
  • Refeyn TwoMP (mass photometry)
  • Beckman Coulter XL-I (AUC)
  • JASCO J-815 (CD)
  • DynaPro Plate Reader III (DLS)
  • LUMICKS C-Trap Dymo-400 (optical tweezers)
Core Facility

NMR

High-field NMR for structure, dynamics, and interaction studies of proteins and nucleic acids.

  • Bruker Avance Neo 600 MHz
  • Varian INOVA 600 MHz (cold probe)
  • Varian 900 MHz Direct Drive (cold probe, Z-gradient)
  • Varian 800 MHz Direct Drive (at CU Boulder)
Core Facility

Mass Spectrometry — Proteomics

Protein identification and quantification, plus structural analysis by chemical crosslinking.

  • Orbitrap Fusion Lumos · Easy-nLC 1200
  • Bruker timsTOF Pro · Evosep One
  • Bruker timsTOF SCP · nanoElute