Resources

Protocols

Bench procedures we use routinely and are happy to share.

Software & tools

Open-source tools written in the lab, plus resources we maintain for the wider community.

ProteinCHAOS

MD-inspired generative art tool for visualising protein dynamics.

External ↗

Core Facilities at CU Anschutz

Our structural and biophysical work is supported by the School of Medicine's shared Structural Biology and Biophysics core facilities. We routinely access the resources below — follow each link for instrumentation details, rates, and booking.

Core Facility

Cryo-EM Structural Biology Shared Resource

Single-particle cryo-EM from grid screening through automated high-resolution data collection, with remote microscope control.

  • Talos Arctica 200 kV X-FEG (Autoloader)
  • Gatan K3 Summit direct detector
  • Talos L120C 120 kV screening TEM
  • CETA CMOS camera
  • Vitrobot Mark IV
  • Gatan Solarus 950 plasma cleaner
  • Leginon · Appion · cryoSPARC Live
Core Facility

X-Ray Crystallography

Crystallisation screening and optimisation, in-house diffraction data collection, processing, and structure determination.

  • Rigaku MicroMax-007 HF microfocus source
  • VariMax optics · AFC11 goniometer
  • Pilatus 200K area detector
  • Oxford Cobra cryo-system
  • SPT Labtech mosquito Xtal-3 · Phoenix · Alchemist
  • Minstrel imaging · CrystalTrak
  • HKL-3000R
Core Facility

Biophysics

Binding, thermodynamics, oligomeric state, and single-molecule characterisation of proteins and complexes.

  • Biacore T200 (SPR)
  • MicroCal iTC200 (ITC)
  • Monolith NT.115 Pico (MST)
  • Refeyn TwoMP (mass photometry)
  • Beckman Coulter XL-I (AUC)
  • JASCO J-815 (CD)
  • DynaPro Plate Reader III (DLS)
  • LUMICKS C-Trap Dymo-400 (optical tweezers)
Core Facility

NMR

High-field NMR for structure, dynamics, and interaction studies of proteins and nucleic acids.

  • Bruker Avance Neo 600 MHz
  • Varian INOVA 600 MHz (cold probe)
  • Varian 900 MHz Direct Drive (cold probe, Z-gradient)
  • Varian 800 MHz Direct Drive (at CU Boulder)
Core Facility

Mass Spectrometry — Proteomics

Protein identification and quantification, plus structural analysis by chemical crosslinking.

  • Orbitrap Fusion Lumos · Easy-nLC 1200
  • Bruker timsTOF Pro · Evosep One
  • Bruker timsTOF SCP · nanoElute